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U18. Nanotoxicology Unit

U18. Nanotoxicology Unit

U18-E03. Dissection hoods

Dissection hoods.

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U18-E02. Autoclaves, refrigerators, freezers and liquid nitrogen tanks

Autoclaves for sterilization of buffers, media or material (tips, tubes, eppendorfs,…). Refrigerators, freezers (-20ºC and -80ºC) and liquid nitrogen tanks for the preservation of reagents, samples and cells.

Autoclaves

Refrigerators and -20ºC freezers

 Freezers (-80ºC)

Freezers (-80ºC)

Nitrogen liquid tanks

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U18-E01. Cell culture rooms

Four cell culture rooms fully with equipped with cell culture hoods, CO2 incubators, automatic cell counters, refrigerators, freezers, baths, centrifuges, inverted fluorescence and phase contrast microscopes.

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U18-S01. In vitro Nanotoxicology (Remote) OUTSTANDING

Assays of cell viability/toxicity by XTT, release of LDH and similar indicators.

  • Study of toxicity in normal human cells immortalized by viral infection or tTERT, by means of metabolic XTT test,
  • Production of reactive oxygen species (ROS ) by fluorimetry.

Internalization assays

  • Study of the mechanism of toxic action
  • Analysis of hemocompatibility in blood
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U18-S02. In vivo Nanotoxicology, preclinical histopathology in animal models (Remote) OUTSTANDING

Sample preparation, inclusion, section and staining for preclinical histology (paraffin and frozen samples) as well as anatomopathological evaluation.

Complete necropsy:

  • Hematology
  • ADME/Tox of nanoparticles
  • Study of distribution of markers of interest by immunohistochemistry.
  • Study of the mechanism of toxic action (apoptosis, caspase-independent death, autophagia, necrosis).
  • Preclinical toxicity assays in animal models.

Product characterization studies, solubility and other properties studies; Inmunoassay techniques; Molecular biology techniques; Cell culture techniques; Flow cytometry; Safety studies: Nervous, cardiovascular and respiratory systems; Toxicology studies; PK/PD studies, Security testing of advanced therapies using small and large animals models.

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U18-S03.  In vivo TOXICOLOGY (Onsite&Remote) OUTSTANDING

Complete necropsy:

  • Hematology
  • ADME/Tox of nanoparticles
  • Study of distribution of markers of interest by immunohistochemistry.
  • Study of the mechanism of toxic action (apoptosis, caspase-independent death, autophagia, necrosis).
  • Preclinical toxicity assays in animal models
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